anti-mouse cd105-pe-cy7  (Thermo Fisher)

Journal: International Journal of Molecular Sciences

Article Title: Photobiomodulation Enhances the Angiogenic Effect of Mesenchymal Stem Cells to Mitigate Radiation-Induced Enteropathy

doi: 10.3390/ijms20051131

Figure Lengend Snippet: Photobiomodulation (PBM)-preconditioned mesenchymal stem cells (MSCs) restore the microvasculature of the irradiated intestine. ( A ) Immunohistochemical analysis of CD31 in the small intestinal tissue of control (CON), irradiated (IR), IR and MSC-treated (IR+MSC), and IR and PBM-preconditioned MSC-treated (IR+PBM-MSC) groups. Scale bar = 50 μm. Arrowheads indicate a CD31-positive area. ( B ) Flow cytometric analysis to enumerate the endothelial cells in the isolated lamina propria of CON, IR, IR+MSC, and IR+PBM-MSC groups. ( C ) Total endothelial cells in the isolated lamina propria were determined by CD31-positive cells. ( D ) Angiogenesis in the isolated lamina propria was determined by CD31 and CD105-double positive cells. Data are presented as the mean ± SEM; n ≥ 3 per group. * p < 0.05 compared to the control; # p < 0.05 compared to the IR group.

Article Snippet: After centrifugation, cells were re-suspended in PBS containing 2% BS and 2 mM of EDTA and subjected to staining with APC or phycoerythrin–cyanine 7 (PE-Cy7)-conjugated anti-mouse CD31 and CD105 antibodies (eBioscience) for flow cytometry.

Techniques: Irradiation, Immunohistochemical staining, Control, Isolation